Super-Resolution

THOR - Nikon A1 laser scanning, structured illumination and TIRF (Porter B047A)

Pic
Thor

Ìý

Lasers:

Launch: LUNV6, high-output

Excitation at: 405, 445, 488, 514, 561, 647 (nm)

Emission (Chroma) and molecules imaged:

  • Emission filter: 425-475 (nm): DAPI, Hoechst, DyLight-405, Alexa405, etc.
  • Emission filter: 467.5-502.5 (nm): CFP, cerulean, etc.
  • Emission filter: 500-550 (nm): Alexa488, FITC, EGFP, Opal520, etc.
  • Emission filter: 521.5-554.5 (nm): YFP, Venus, etc.
  • Emission filter: 575-625 (nm): Alexa555, 568, 594, TxRed, mRFP, mCherry, TdTomato, Opal570, etc.
  • Emission filter: 650-720 (nm): Alexa647, Cy5, Opal620, etc.
  • DIC imaging possible (laser scanning confocal only)

Filters for widefield:

DAPI, GFP, RFP, Brightfield

N-SIM super-resolution lens:

  • 100x 1.49NA OIL SR Apo TIRF

Additional standard objectives:

  • 4x 0.2NA Plan ApoÌýλ WD 20 (mm)
  • 10x 0.45NA Plan Apo λ WD 4 (mm)
  • 20x 0.75NA Plan Apo VC λ WD 1 (mm)
  • 40x 0.95NA Plan Apo λ WD 0.25-0.17 (mm)
  • 60X 1.27NA Water SR Plan Apo WD 0.17 (mm)

Available upon request:Ìý

  • 60x 1.40NA Plan Apo OIL VC λ WD 0.13 (mm)
  • inquire

Laser Scanning Confocal:

  • 2 x GaAsP high-sensitivty PMTs (CFP, GFP, YFP, RFP)
  • 2 x multi-alkali PMTs (DAPI, far-red)

N-SIM:

  • Andor iXON 897 Ultra EMCCD (512x512)

Ìý

Ìý

Ìý

  • 3D structured illumination, 'fast' 2D SIM
  • TOKAI-HIT Cell Incubator 1 x 35 (mm) dish, chamber side (coverslip bottom), multiwell plate
  • Motorized X, Y, Z and separate MCL Piezo Z with 200 micron range
  • Multi-point, time-lapse, optical sectioning, large-area montage with stitching
  • Advanced analysis tools via separate workstation, including spectral deconvoltion, and full analysis suite (inquire with Director)

  • SIM imaging with 647nm laser:

    Thor, SIM, far red
  • ÌýAR 5.04; 64 bit
  • Please contact Director for training

Nikon Ti2E AXR NSPARC (Porter B049)

Pic
AX NSPARC front view

Lasers:

Launch: LUD-S4Ìý

Excitation at: 405, 488, 561, 640 (nm)

Emission (Chroma) and molecules imaged:

  • Emission filter: 425-475 (nm): DAPI, Hoechst, DyLight-405, Alexa405, etc.
  • Emission filter: 500-550 (nm): Alexa488, FITC, EGFP, Opal520, etc.
  • Emission filter: 575-625 (nm): Alexa555, 568, 594, TxRed, mRFP, mCherry, TdTomato, Opal570, etc.
  • Emission filter: 650-720 (nm): Alexa647, Cy5, Opal620, etc.
  • DIC imaging possible (laser scanning confocal only)

Light source and filters for viewing by eye:

• Nikon D-LEDI; 385, 475, 550, 621 nm for DAPI, GFP, RFP, Cy5 with multi-band emitter, brightfield

NSPARC super-resolution lens:

  • 60X PLAN APO TIRF oil NA 1.49 WD 0.12 (mm), DIC

Additional standard objectives:

  • 2x PLAN APO Ìýλ NA 0.10, WD 8500 (mm)
  • 10X PLAN APO λ D NA 0.45 WD 4.0 (mm), DIC
  • 20X CFI60 PLAN APO λ D ÌýNA 0.80 WD 0.80 (mm), DIC
  • 40XC PLAN APO λ S ÌýSILICONE NA 1.25 WD 0.30 (mm), DIC, correction collar (23-37 degrees C)
  • 60X PLAN APO IR SR water 1.27NA WD 0.18 (mm), DIC

Available upon request:

  • 60x 1.40NA Plan Apo OIL VC λ WD 0.13 (mm)
  • 40x NA 0.95 WD 0.18 (mm)
  • inquire
  • DUX-VB, 4 PMT-GA GaAsP
  • Nikon Spatial Array Confocal (NSPARC) pinhole array sensor
  • High frame-rate imaging using resonance scanner
  • Super resolution to 100nm XY using NSPARC detector
  • Adjustable GFP and RFP emission bandwidth (confocal only)
  • TOKAI-HIT Cell Incubator 1 x 35 (mm) dish, chamber side (coverslip bottom), multiwell plate
  • Motorized X, Y, Z and separate Prior QGNPS083-NI Piezo 600 micron Z-stage
  • Multi-point, time-lapse, optical sectioning, large-area montage with stitching
  • Advanced analysis tools via separate HP Z4 workstation, including spectral deconvolution, and full analysis suite (inquire with Director)
  • NIS Elements AR 6.02.03 with JOBS, general analysis

in progress